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    Cloning and characterization of a Mimulus lewisii NPR1 gene involved in regulating plant resistance to Rhizoctonia solani

     

    论文题目

    Cloning and characterization of a Mimulus lewisii NPR1 gene involved in regulating plant resistance to Rhizoctonia solani

    作    者

    Xia He1, Yancai Shi2,*

    发表期刊

    Plant Biotechnology

    //

    2018

    摘    要

    The monkey flower Mimulus lewisii is a new emerging model plant for the study in corolla tube formation,pigmentation patterns and pollinator selection, etc. However, the cultivation and management of this plant are difficult dueto its susceptibility to a wide range of pathogens and the lack of rigid varieties with high levels of resistance to pathogens. Inthis regard, genetic engineering is a promising tool that may possibly allow us to enhance the M. lewisii disease resistanceagainst pathogens. Here, we reported the isolation and characterization of non-expressor of pathogenesis related gene 1(NPR1) gene from M. lewisii. The phylogenetic tree constructed based on the deduced sequence of MlNPR1 with homologsfrom other species revealed that MlNPR1 grouped together with other known NPR1 proteins of Scrophulariaceae family,and was nearest to Mimulus guttatus. Furthermore, expression analysis showed that MlNPR1 was upregulated after SAtreatment and fungal infection. To understand the defensive role of this gene, we overexpressed MlNPR1 in M. lewisii. Thetransgenic lines showed slight phenotypic abnormalities, but constitutive expression of MlNPR1 activates defense signalingpathways by priming the expression of antifungal PR genes. Moreover, MlNPR1 transgenic lines showed enhanced resistanceto Rhizoctonia solani there was delay in symptoms and reduced disease severity than non-transgenic plants. Altogether, thepresent study suggests that increasing the expression level of MlNPR1 may be a promising approach for development ofmonkey flower cultivars with enhanced resistance to diseases.

     


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